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Objective To explore the FOXP3-related mechanism underlying head and neck squamous cell carcinoma.Methods We used cbioportal to identify the co-expressed genes of FOXP3 in 279 samples from head and neck squamous cell carcinoma in TCGA database.We used String database to establish the co-expression network of FOXP3.The function of co-expression network was identified through DAVID database.We used miRTarBase and StarBase database to screen the microRNA,lncRNA and circRNA that regulate FOXP3.Finally,Cytoscape software was used to establish FOXP3-related ceRNA network.Results We found 950 FOXP3 related co-expressed gene.(Spearman score over 0.5) These genes were enriched in immune response including T cell,leukocyte and lymphocyte activation.CeRNA network revealed that 2 microRNAs (i.e.,miR-31-5p and miR-210-3p),42 lncRNAs (e.g.,XIST,TUG1,JRK and LINC00473) and 31 circRNAs (e.g.,ZNF223 _hsa_ circ_ 000898 and ISY1 _hsa _circ _001090) could regulate FOXP3.Conclusion We established FOXP3-related ceRNA network and identified 42 lncRNAs and 31 circRNAs that regulate FOXP3.The data generated from this study could provide a new cut point in research and treatment of head and neck squamous cell carcinoma.
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Objective Screening the functional gene modules that can play important roles in hypopharyngeal cancer and the potential anti-cancer drugs.Methods GEO database and MeV software were employed to screen the differentially expressed genes in hypopharyngeal cancer.Using STRING database,the protein-protein interaction network was identified.MCODE plugin of Cytoscape was used to identify the functional gene modules in the network.Based on DAVID database,the functions of modules were identified.DrugBank was used to screen the potential drugs that regulate the target genes of modules.Results 1 222 differentially expressed genes including 219 interaction pairs were i-dentified in whole genome profiling(P <0.05 ).Seven functional modules were identified in the network.The results of function analysis showed the module genes were enriched in cancer development related-function ‘regulation of angiogenesis’,‘cell adhesion’,‘DNA meta-bolic process’.A total of 50 potential drugs that regulating the 5 modules were screened.Conclusion Five functional modules that regulate the progress of hypopharyngeal cancer were identified,and maybe they can promote hypopharyngeal cancer through some functions such as regulation of angiogenesis 18 up-regulated protein kinases were identified.Their kinase inhibitors may potential have a role in anti-cancer, which provides a new target point for molecular therapy of nasopharyngeal cancer.
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Objective To investigate telomerase reverse transcriptase (TERT ) and acticator protein 1(AP -1) expression and it's corroloation in laryngeal carcinoma tissue .Methods 24 human laryngeal carcinoma tissue were analised by RT -PCR and quantum -dot based immunofluorescence assay for the expression of TERT and AP -1 . Results The expression of TERT mRNA and the expression of c -Fos ,c-Jun in laryngeal carcinoma were postive‐ly related .Correlation coefficient was 0 .574 and 0 .809 ,respectivly(P<0 .01) .Conclusion TERT and AP -1 were expressed at high levels and positively correlated in human laryngeal carcinoma .
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OBJECTIVE@#To investigate the clinicopathological significance of the cyclin E expression in laryngeal squamous cell carcinomas (LSCCs).@*METHOD@#The expression of cyclin E was detected by immunohistochemistry method in 50 cases of LSCCs, 20 cases of dysplastic squamous epithelium and 10 polyps of vocal cord.@*RESULT@#The positive expression of cyclin E was localized in the nuclei of cells. The positive rate of cyclin E protein in LSCCs, dysplastic squamous epithelium and polyps of vocal cord was 10% (1/10), 15% (3/20), 6% (3/50), respectively. There's no significant difference between them. While the expression of cyclin E in laryngeal cancer stromal vascular endothelial cells was significantly related with clinical stage and lymph node metastasis (P<0.01).@*CONCLUSION@#In LSCCs, the expression of cyclin E has non-specificity, probably it has no participate the malignant transform events of laryngeal cancer cells. However, cyclin E is sensitive to vasculogenesis relatively; the positive expression of cyclin E may evaluate the malignant phase of LSCCs indirectly.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , Cyclin E , Metabolism , Laryngeal Neoplasms , Metabolism , Pathology , Neoplasm Staging , PrognosisABSTRACT
OBJECTIVE@#The aim of this study was to compare the sensitivity of conventional culture and PCR method.@*METHOD@#DNA samples were collected from the sinus of 20 patients with chronic sinusitis and 18 patients diagnosed as fungal sinusitis clinically. Fungal specific PCR analysis and standard culture were performed on every sample. chi2 analysis was used to determine the statistical differences between groups.@*RESULT@#Fungal DNA were detected in 78% of patients with fungal sinusitis and 5% of patients with chronic sinusitis in PCR analysis, while the positive rate were 44% and 0% in standard cultures respectively.@*CONCLUSION@#PCR is more rapid and sensitive than nasal swab culture in detecting the fungi in sinus mucosa. Our study suggests that it is an efficient method in diagnosis of fungal sinusitis.